Rapid plasmid insert amplification with polymerase chain reaction
نویسندگان
چکیده
منابع مشابه
SPECIFIC AMPLIFICATION OF ASPERGILLUS FUMlGATUS DNA BY POLYMERASE CHAIN REACTION
Invasive aspergillosis (1 is a life-threatening condition in immunocompromised patients. An early diagnosis is of great importance because early treatment may resolve this potentially fatal infection. Recently, the polymerase chain reaction (PCR) has been used successfully in detecting specific DNA of several pathogen. In this study, nested PCR was used to detect DNA specific for A!.pergiflus s...
متن کاملUnusual amplification in polymerase chain reaction for a plasmid containing an insert derived from bovine genomic DNA
The saliva of various animals contains prolinerich proteins which may play important roles in prevention of mineral precipitation, protection of dietary and digestive proteins from interaction with tannins, and modulation of bacterial colonization on the tooth surface. Previously, we found a segment of Escherichia coli genomic DNA in bovine tooth germ mRNA encoding the prolinerich protein P-B. ...
متن کاملPolymerase Chain Reaction and Qf3 Replicase Amplification
The polymerase chain reaction (PCR) and Q/3 replicase are two methods in which nucleic acid polymerases are used for amplification. Although these approaches share many similar problems concerning target contamination and probe specificity, they differ dramatically in their mechanisms of action and modes of application.The PCR method amplifies target sequences between two priming oligonucleotid...
متن کاملspecific amplification of aspergillus fumlgatus dna by polymerase chain reaction
invasive aspergillosis (1 is a life-threatening condition in immunocompromised patients. an early diagnosis is of great importance because early treatment may resolve this potentially fatal infection. recently, the polymerase chain reaction (pcr) has been used successfully in detecting specific dna of several pathogen. in this study, nested pcr was used to detect dna specific for a!.pergiflus s...
متن کاملPlasmid Copy Number Determination by Quantitative Polymerase Chain Reaction
Recombinant therapeutic proteins are biopharmaceutical products that develop rapidly for years. Recombinant protein production in certain hosts requires vector expression harboring the gene encoding the corresponding protein. Escherichia coli is the prokaryote organism mostly used in recombinant protein production, commonly using a plasmid as the expression vector. Recombinant protein productio...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1988
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/16.8.3579